Bulk packings
YMC*GEL silica-based HPLC preparative packing materials offer a wide range of functional groups, from reversed phase and normal phase to particle sizes ranging from high-resolution 3 um materials to 150 um materials for open-column chromatography. YMC*GEL HG uses high-density, high-strength silica gel, so particles do not break even after repeated packing and unpacking. High-performance and cost-effective YMC*GEL / YMC*GEL HG packing materials are widely used both in Japan and overseas.
YMC*GEL HG uses high-density silica gel with excellent mechanical strength. Even after more than ten repacking cycles into dynamic axial compression columns, particle breakage and column-pressure increase are not observed, allowing repeated use.
| Packing conditions | |
| Column size | 100 X 50 mm I.D. |
|---|---|
| Packing pressure | 6.5 MPa |
| Column performance test | |
| Eluent | methanol/water (85/15) |
| Flow rate | 50 mL/min |
YMC*GEL / YMC*GEL HG offers a wide range of functional groups, including wide-pore packing materials, and is suitable for separating peptides such as insulin and proteins. Because YMC*GEL HG has selectivity equivalent to analytical YMC-Pack columns, scale-up from analysis to preparative purification is straightforward.
| Analytical conditions (1), (3) | |
| Column | YMC-Pack C8 (5 um, 20 nm) 150 X 4.6 mm I.D. |
|---|---|
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 27%B (0-5 min) 27-40%B (5-20 min) |
| Flow rate | 0.8 mL/min |
| Temperature | ambient |
| Detection | UV at 220 nm |
| Preparative conditions (2) | |
| Column | YMC*GEL C8 (10 um, 20 nm) 150 X 50 mm I.D. |
|---|---|
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 27%B (0-5 min) 27-40%B (5-20 min) |
| Flow rate | 100 mL/min |
| Temperature | ambient |
| Detection | UV at 220 nm |
| Packing material | Particle size (um) | Pore size (nm) | Features |
|---|---|---|---|
| ODS-A | 3, 5, 75, 150 | 12, 20, 30 |
|
| ODS-A-HG | 7, 10, 15, 20, 50 | ||
| ODS-AM | 3, 5 | 12 |
|
| ODS-B | 5, 10, 15, 20, 50 | 20 |
|
| C8 | 3, 5, 10, 15, 20, 50 | 12, 20, 30 |
|
| Phenyl | 5, 10, 15, 20, 50 | 12 |
|
| CN | 5, 10, 15, 20, 50 | 12 |
|
| SIL | 5, 10, 15, 20, 50 | 6, 12, 20 |
|
| Packing material | Particle size (um) | Pore size (nm) | Features |
|---|---|---|---|
| YMC*GEL ODS-A | 50, 75, 150 | 12, 20 | Reversed-phase open-column purification |
| YMC*GEL SIL | 50, 75, 150 | 6, 12 | Normal-phase open-column purification |
